Control of invasive grass carp (Ctenopharyngodon idella) populations in the Western Lake Erie Basin merits adaptive management guided by the best available science. Presently (2024), capture of mature grass carp in rivers during spawning season is most efficient, so knowing when and where grass carp are spawning is essential information for natural resource agencies. Using bidirectional drift modeling and grass carp ichthyoplankton samples captured in the Maumee River during the 2017–2019 spawning seasons, this study identified 12 probable grass carp spawning areas in the lower 96.5-kilometers of the Maumee River. These spawning areas were located both above and below the Grand Rapids/Providence low-head dams. Three areas showed evidence of multiyear use, while nine had multi-event use. Spawning activity had no definitive diel variation and occurred at an average photoperiod of 15.15 h. The maturation metric ADD15, or annual degree days above 15 degrees Celsius, generally exceeded the 655 threshold for spawning; however, some spawning occurred when ADD15 ≤235, indicating spawners likely matured in a warmwater discharge. The probable spawning areas were generally characterized by mean velocities between 0.4 and 2.1 m per second (with locally higher velocities possible), areas of high turbulence produced by dam spillways or bedrock outcroppings, channel constrictions, confluences, islands, and bridges with piers in the water. Spawning suitability indices (SSI), based on velocity, varied considerably between spawning areas and SSI models. These results could be used to inform control efforts and predict potential grass carp spawning locations in other rivers under threat of invasion.